human tc cell lines tpc 1 cl 0643  (Procell Inc)

Journal: Extracellular Vesicles and Circulating Nucleic Acids

Article Title: A single-sEV analysis identifies plasma EPCAM + sEVs as a biomarker for early diagnosis and monitoring postoperative remission of thyroid cancer

doi: 10.20517/evcna.2025.93

Figure Lengend Snippet: The impact of TC-derived sEVs on TC development in vitro and in vivo . The effects of TC-derived sEVs on TC cell proliferation determined by CCK-8 assays (A and B) or colony formation assays (C and D); Representative images of migration assays (E and F) and invasion assays (G and H) of TC cells subjected to PBS or plasma sEVs from TC patients; (I) Schematic diagram of mouse experiments; (J) TPC-1 tumors were photographed when the tumor-bearing mice were sacrificed; (K) The tumor volume was compared between two groups. The data was analyzed using the Student’s t -test. * P value < 0.05; ** P value < 0.01; *** P value < 0.001; ns: not significant. TC: Thyroid carcinoma; sEVs: small extracellular vesicles; CCK-8: cell counting kit-8; PBS: phosphate-buffered saline; OD450: optical density at 450 nm.

Article Snippet: Human TC cell lines TPC-1 and BCPAP were purchased from Procell Life Science & Technology Co., Ltd. and Fuheng Biology, respectively, and they were authenticated using short tandem repeat (STR) method before experiment.

Techniques: Derivative Assay, In Vitro, In Vivo, CCK-8 Assay, Migration, Clinical Proteomics, Cell Counting, Saline

Journal: Extracellular Vesicles and Circulating Nucleic Acids

Article Title: A single-sEV analysis identifies plasma EPCAM + sEVs as a biomarker for early diagnosis and monitoring postoperative remission of thyroid cancer

doi: 10.20517/evcna.2025.93

Figure Lengend Snippet: The function of EPCAM + sEV. (A) The western blotting analysis of EPCAM in 293T cells stably transfected with shNC or shEPCAM. The sEVs were isolated from 293T cells stably transfected with shNC or shEPCAM, namely shNC-sEV or shEPCAM-sEV, respectively. TPC-1 and BCPAP cells were treated with shNC-sEV or shEPCAM-sEV; (B and C) TC cell proliferation determined by CCK-8 assays; (D and E) TC cell proliferation determined by colony formation assays; Representative images of migration assays (F and G) and invasion assays (H and I) of TC cells subjected to shNC-sEV or shEPCAM-sEV. The data was analyzed using the Student’s t -test. * P value < 0.05; ** P value < 0.01; *** P value < 0.001; ns: not significant. EPCAM: Epithelial cell adhesion molecule; sEVs: small extracellular vesicles; shNC: lentivirus of negative control short hairpin RNA; shEPCAM: lentivirus of EPCAM short hairpin RNA; TC: thyroid carcinoma; CCK-8: cell counting kit-8; OD450: optical density at 450 nm.

Article Snippet: Human TC cell lines TPC-1 and BCPAP were purchased from Procell Life Science & Technology Co., Ltd. and Fuheng Biology, respectively, and they were authenticated using short tandem repeat (STR) method before experiment.

Techniques: Western Blot, Stable Transfection, Transfection, Isolation, CCK-8 Assay, Migration, Negative Control, shRNA, Cell Counting